A1013
Title: Sceptic: Pseudotime analysis for time-series single-cell sequencing and imaging data
Authors: Gang Li - Changping Laboratory (China) [presenting]
Abstract: Several computational methods have been developed to construct single-cell pseudotime embeddings for extracting the temporal order of transcriptional cell states from time-series scRNA-seq datasets. However, existing methods suffer from low predictive accuracy, and this problem becomes even worse when generalized to other data types such as scATAC-seq or microscopy images. To address this problem, Sceptic is proposed, a support vector machine model for supervised pseudotime analysis. It is demonstrated that Sceptic achieves significantly improved prediction power relative to state-of-the-art methods, and that Sceptic can be applied to a variety of single-cell data types, including single-nucleus image data.
